This paper establishes an evolutionary game model to analyze the firm migration promoted by policy guidance, and probes the evolutionarily stable strategy (ESS) between the undertaking governments and migrating enterprises. Results show that, firstly, the increasing profit margins obtained after migration, firm migration costs, undertaking governments incentives, and the environmental regulation differences between emigration and undertaking regions are important factors affecting firm migration; secondly, the key factors of migration among different types of enterprises exist obvious differences in different situations. Furthermore, this paper suggestions that the enterprises, which have lost comparative advantages in coastal areas, may orderly migrate to the Midwest areas of China.